Journal: The Journal of Infectious Diseases

Article Title: Role of the Gut Microbiota of Children in Diarrhea Due to the Protozoan Parasite Entamoeba histolytica

doi: 10.1093/infdis/jiv772

Figure Lengend Snippet: Characteristics of the Study Population, by Entamoeba histolytica Infection Status During the First 2 Years of Life

Article Snippet: Positive stool samples were retested with the E. histolytica species-specific E. histolytica II enzyme linked immunosorbent assay (ELISA; TechLab) [ ].

Techniques: Infection

Journal: The Journal of Infectious Diseases

Article Title: Role of the Gut Microbiota of Children in Diarrhea Due to the Protozoan Parasite Entamoeba histolytica

doi: 10.1093/infdis/jiv772

Figure Lengend Snippet: Characteristics of 307 Children With an Initial Entamoeba histolytica Infection

Article Snippet: Positive stool samples were retested with the E. histolytica species-specific E. histolytica II enzyme linked immunosorbent assay (ELISA; TechLab) [ ].

Techniques: Infection

Journal: The Journal of Infectious Diseases

Article Title: Role of the Gut Microbiota of Children in Diarrhea Due to the Protozoan Parasite Entamoeba histolytica

doi: 10.1093/infdis/jiv772

Figure Lengend Snippet: Characteristics of 54 Children With a Third Entamoeba histolytica Infection

Article Snippet: Positive stool samples were retested with the E. histolytica species-specific E. histolytica II enzyme linked immunosorbent assay (ELISA; TechLab) [ ].

Techniques: Infection

Journal: The Journal of Infectious Diseases

Article Title: Role of the Gut Microbiota of Children in Diarrhea Due to the Protozoan Parasite Entamoeba histolytica

doi: 10.1093/infdis/jiv772

Figure Lengend Snippet: Children with fecal anti- Entamoeba histolytica Gal/GalNAc lectin immunoglobulin A (IgA) had a lower rate of reinfection. The y -axis denotes the percentage of children with an E. histolytica infection (n = 307), and the x -axis denotes the interval between the first and second E. histolytica infections. A statistically significant increase in the time to reinfection was observed for children who were fecal IgA anti-lectin positive (red line), compared with those who were negative (black line; P = .0175, by the log-rank [Mantel–Cox] test). A total 65 children tested positive and 242 tested negative for E. histolytica IgA.

Article Snippet: Positive stool samples were retested with the E. histolytica species-specific E. histolytica II enzyme linked immunosorbent assay (ELISA; TechLab) [ ].

Techniques: Infection

Journal: The Journal of Infectious Diseases

Article Title: Role of the Gut Microbiota of Children in Diarrhea Due to the Protozoan Parasite Entamoeba histolytica

doi: 10.1093/infdis/jiv772

Figure Lengend Snippet: Increased parasite burden with diarrhea in first but not second year of life. The amount of Entamoeba histolytica in stool specimens from children who had diarrhea and those who were asymptomatic was determined by quantitative polymerase chain reaction (qPCR). Samples collected from an E. histolytica –associated diarrheal sample (DS) or a sample in which E. histolytica was detected but colonization was not coincident with disease (COL) during the first infection ( A ) and second infection ( B ). The mean and standard error are shown. The y -axis indicates the threshold value of the qPCR assay specific for E. histolytica . As the quantification cycle distribution was not Gaussian, Kruskal–Wallis 1-way analysis of variance was used to determine that this data set contained significant differences. The Dunn multiple comparison test was used to determine that the year 1 data were significantly different from each other. * P ≤ .05 and ** P ≤ .01.

Article Snippet: Positive stool samples were retested with the E. histolytica species-specific E. histolytica II enzyme linked immunosorbent assay (ELISA; TechLab) [ ].

Techniques: Real-time Polymerase Chain Reaction, Infection, Comparison

Journal: The Journal of Infectious Diseases

Article Title: Role of the Gut Microbiota of Children in Diarrhea Due to the Protozoan Parasite Entamoeba histolytica

doi: 10.1093/infdis/jiv772

Figure Lengend Snippet: Expansion of Prevotella copri was associated with diarrhea due to Entamoeba histolytica in children from 600 to 800 days of age. Samples collected between 600 and 800 days of age from an E. histolytica –associated diarrheal sample (DS) or a sample in which E. histolytica was detected but colonization was not coincident with disease (COL) were compared after normalization to Enterobacteriaceae levels. The y -axis denotes the P. copri qPCR signal normalized to the Enterobacteriaceae burden, as determined by 16S bacteria ribosomal DNA load (log), and the x -axis denotes the specimen type. ** P ≤ .01.

Article Snippet: Positive stool samples were retested with the E. histolytica species-specific E. histolytica II enzyme linked immunosorbent assay (ELISA; TechLab) [ ].

Techniques: Bacteria

Journal: The Journal of Infectious Diseases

Article Title: Role of the Gut Microbiota of Children in Diarrhea Due to the Protozoan Parasite Entamoeba histolytica

doi: 10.1093/infdis/jiv772

Figure Lengend Snippet: Characteristics of 162 Children With a Second Entamoeba histolytica Infection

Article Snippet: Positive stool samples were retested with the E. histolytica species-specific E. histolytica II enzyme linked immunosorbent assay (ELISA; TechLab) [ ].

Techniques: Infection